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yard Negative Unknown Upon being given a bacteriuml elaboration containing two bacterium, I was assigned the task of change the several(prenominal) bacterium. The two bacteria of the mixture were angiotensin-converting enzyme one thousandme dictatorial and one gigabyte negative. The experiment has two split and this is the donation of isolating the gram-negative bacteria. The technique used was called streaking for closing outwit through in which the bacteria were streaked thinly on food for thought agar in different directions in a normal that left respective(prenominal) colonies on the last growth streak. These individual colonies could be identified by their individual characteristics such as color and shape and distinguished from one another. This was not tardily with this horti finis because both(prenominal) bacteria grew in colonies that were small, white, and shaped similar. To sieve these bacteria I had to first gram stain the bacterial mixture to see if I had both types of bacteria and so I used the following locomote to perform the gram stain. 1. The bacteria are discolourationed on a slide and allowed to dry. 2. The bacteria is heat fixed to the slide 3. vitreous silica violet pigment is added to the smear for 1 minute 4. washing the pigment attain with distilled peeing 5. Apply Grams Iodine for 1 minute 6. Wash iodine off with distilled water 7.

Decolorize the smear with 95% ethyl spirituous beverage hurtle by drop until it runs off give 8. Wash off alcohol with distilled water 9. Counterstain with Safranin for 45 seconds 10. Wash of f Safranin with distilled water 11. Blot d! ry with Bibulous paper Following this test, I found that I had a lot more gram negative bacteria than the gram positive and so I could easily latch on one colony of my streaked culture and have a high chance of growing a pure culture from one of the isolated colonies. So, with this method, I grew a pure culture of the gram negative bacteria that I would use in my tests to determine its identity. I ran several tests on the bacteria staring with the Gram stain that proved that the bacteria were gram negative....If you wishing to get a full essay, couch it on our website: OrderCustomPaper.com

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